Fig 1.
Distribution of naturally occurring Shope papillomas of cottontail rabbits as described by Shope.
Redrawn following Kreider, 1981 [13]. The locality of prior PCR positive samples (pink dots) and 16 of 18 localities of symptomatic samples tested in this study (black dots) for which geographical information was available are shown. Three samples were collected from the same locality (Lexington, Nebraska).
Fig 2.
Sylvilagus floridanus voucher specimens with typical SfPV horn-like growths.
Left, one of the 13 PCR positive specimens; right, specimen not tested to preserve its integrity. Specimens from the University of Kansas Natural History Museum (KU) collection.
Table 1.
Results of tests on symptomatic Sylvilagus rabbits. Includes locality and year of host collection.
Table 2.
List of Leporidae voucher specimens inspected for SfPV growths in the Kansas University Natural History Museum.
Fig 3.
Maximum likelihood tree of partial E7 SfPV 1 sequences.
The tree was constructed using six 153 bp E7 sequences obtained in our study (1R, 2R, etc.), supplemented with six sequences from previous studies (GenBank accession numbers shown), along with locality and date. Host species is indicated by color (blue = S. audubonii, red = S. floridanus, white = Sylvilagus sp.). Numbers indicate bootstrap support for internal nodes (with 1000 repetitions). Partial sequence 16R was manually added to its closest relatives (resolved sequence was 100% identical to K02708 and AJ404003), but it was excluded from the bootstrap analysis. The assumed position of sequence 16R is represented with a dashed line.
Fig 4.
Distribution of the SfPV1 cases and three known Sylvilagus hosts.
Host distributions are depicted with different shading [16]. Similarly SfPV case locations are color coded for host species, etc. See legend for details. Outer circles to points indicate SfPV positive samples, their absence indicates symptomatic individuals. We omit the locality of one symptomatic S. audubonii and CRPV Hershey “Kansas” (GenBank Acc. No: JF303889), for which locality information was missing or vague.