Fig 1.
Diagram of Hevea epicormic shoots (A) and the cross-sections of bark (B-C) showing the sites of chemical application and secondary laticifer differentiation.
The gray arrow in panel A indicates the site of chemical application and sampling. Panel B, treated with 20 μM coronatine (COR). Panel C, treated with water. EU, extension unit. White arrows show the primary laticifers. Black arrows show the secondary laticifers. Ca, cambium; St, sieve tube. Bars = 100 μm.
Fig 2.
Reverse northern blot analysis of differentially expressed ESTs in the forward and reverse SSH libraries.
Panel A, B and C, hybridization with unsubtracted cDNA probes from samples upon COR treatment for 1 day (A), 2 days (B) and 3 days (C). Panels D, E and F, hybridization with unsubtracted cDNA probes from samples upon water treatment for 1 day (D), 2 days (E) and 3 days (F). a to k, ESTs from the forward SSH library. i to t, ESTs from the reverse SSH library. The strong and corresponding weak hybridization signals were indicated with solid rings and dotted rings, respectively.
Fig 3.
The GO standard analysis of 286 genes from the forward and reverse SSH libraries by BGI WEGO.
Fig 4.
Expression pattern of all 10 unigenes from the forward (A) and reverse (B) SSH library by real-time PCR.
Shoots were treated with 20 μM COR and water. Cambia-containing tissues were collected half an hour (0.5 h), one hour (1 h), two hours (2 h), four hours (4 h), eight hours (8 h), one day (1 d), two days (2 d) and three days (3 d) after treatments. The relative expression was normalized to the housekeeping genes of HbACTIN and HbRH8. The data were shown as averages ± SE. *, significant difference (P < 0.05); **, very significant difference (P < 0.01).