Fig 1.
Genomic in situ hybridization (GISH) identification of the mitotic and meiotic patterns in wheat-P. huashanica disomic addition line 25-10-3.
(a) Somatic chromosomes in the root tips: 2n = 44, i.e., 42 wheat chromosomes and two P. huashanica chromosomes with strong yellowish-green hybridization signals. (b) Chromosome behavior of pollen mother cells during metaphase I, i.e., 2n = 22 II, where the alien chromosome comprises one ring bivalent with a yellowish-green signal.
Fig 2.
Analysis of the Ns genome of P. huashanica using SCAR markers.
(a) RHS23, (b) RHS141; M, marker; 1, P. huashanica; 2, common wheat cv. 7182; 3, 25-10-3. Amplified specific bands in the P. huashanica using and addition line25-10-3 respectively (arrows).
Fig 3.
Analysis of addition line 25-10-3 and its parents, i.e., common wheat cv. 7182 and P. huashanica, using EST-SSR and EST-STS marker.
Amplified specific bands in the addition line in chromosomes 6A, 6B, and 6D, respectively (arrows). EST-SSR marker: (a) BE403550; (b)BE490147; (c)BE500768; (d)BF201597; (e)CD452568; EST-STS marker: (f)SWES2; M, marker; 1, P. huashanica; 2, common wheat cv. 7182; 3, 25-10-3.
Table 1.
P. huashanica Ns genome SCAR markers and the wheat EST-SSR and EST-STS markers used in this study.
Fig 4.
Anatomical observations of spike primordium differentiation obtained by binocular dissecting microscopy for addition line 25-10-3 and its parents, i.e., common wheat cv. 7182 and P. huashanica on different dates.
A1–C1: November 6, 2011; A2–C2: December 6, 2011; A3–C3: January 6, 2012; A4–C4: February 6, 2012; A5–C5: March 6, 2012; A6–C6: April 6, 2012; A7–C7: April 6, 2012. A: P. huashanica, B: common wheat cv. 7182, C: addition line 25-10-3.
Fig 5.
Spike primordium differentiation process.
Horizontal axis: sampling date (October 2010 to July 2013), vertical axis: spike primordium development stage. 1: spike primordium; 2: single ridge; 3: early double ridge; 4: mid-double ridge; 5: later double ridge; 6: lemma primordium; 7: floret primordium differentiation; 8: pistil and stamen primordium differentiation; 9: green anther stage; 10: white anther stage; 11: flowering stage; 12: grain-filling stage; 13: maturity stage.
Table 2.
Growth period of wheat-P. huashanica addition line 25-10-3 and its parents, common wheat cv. 7182 and P. huashanica.
Table 3.
Photosynthetic indices of wheat- P. huashanica addition line 25-10-3 and its parents, common wheat cv. 7182 and P. huashanica.
Table 4.
Agronomic traits of wheat-P. huashanica addition line 25-10-3 and its parents, common wheat cv. 7182 and P. huashanica.