Fig 1.
Molecular masses of PSS after fungal treatment for 20 days.
Grey: Mn; white: Mw. Corresponding controls were inactivated with sodium azide. Bars represent means ± standard deviation for triplicate cultures.
Table 1.
Effect of varied conditions on PSS depolymerisation by G. trabeum
.
Fig 2.
Time course of PSS depolymerisation by whole cells G. trabeum DSM 1398.
Cultures in regular Wetzstein medium (■) were compared to those supplemented with 500 μM 2,5-DMBQ (▲), without iron (♦), and cultures inactivated by sodium azide (control; ●). Symbols represent means ± standard deviations for triplicate cultures; standard deviations smaller than symbol size are not shown.
Fig 3.
SEC elution profiles of samples from culture supernatants.
Samples were taken over the course of incubation of a single representative G. trabeum culture containing ultrafiltrated PSS in regular Wetzstein medium. Samples from the start of cultivation ( — ), and from culture day 7 ( - - ), 14 (∙ ∙ ∙), and 20 ( - ∙ - ) were analysed.
Fig 4.
Hydroxyl radical generation by G. trabeum under varied culture conditions.
Production of TBARS from 2-deoxyribose monitored at 530 nm was used as an indicator. Conditions shown: culture in regular Wetzstein medium (■); azide-inactivated (●); no iron (♦); quinone-supplemented (▲). Symbols represent means ± standard deviation for triplicate cultures; standard deviations smaller than symbol size are not shown.
Fig 5.
Concentration of 2,5-DMBQ in supernatants of actively depolymerising G. trabeum cultures in regular Wetzstein medium.
Symbols represent means ± standard deviation of triplicate cultures.
Fig 6.
Total ion current chromatogram of the extract obtained from culture supernatant.
The sample was taken from a regular culture after 10 days of incubation. The magnified sector indicates the 2,5-DMHQ (no. 1) and the 2,5-DMHQ monoacetate (no. 2) as well as the inferred 2,5-DMBQ (*). The signal no. 3 has the molecular mass 190 g/mol.
Table 2.
Relative molecular mass of PSS after three days of LMS treatment measured by SEC
.1