Fig 1.
Cobitis jadovaensis is distributed in Jadova R., C. bilineata in Zrmanja R., C. dalmatina in Cetina R. Cobitis narentana lives in Neretva R., Mislina, Modro oko lake, Norin R., Trebišnjica R. and Hutovo blato wetland. Cobitis illyrica inhabits Matica R. with Baćinska lakes, as well as Prološko blato and Krenica lakes. C. herzegoviniensis is distributed in Mostarsko blato karstic field.
Table 1.
Sampling localities and number of specimens included in the investigation.
Fig 2.
Divergence time estimations based on the cyt b sequences of the Adriatic spined loaches.
Numbers in squares represent BI posterior probabilities. Timing of the splitting events is presented by mean value and the 95% credibility range (in million years ago).
Table 2.
Genetic polymorphism of the investigated species based on three genetic markers.
Fig 3.
Migration rates among populations of C. narentana and C. illyrica based on mitochondrial (cyt b) and nuclear genes (RAG 1 and S7 first intron).
Bold numbers present maximum likelihood estimation of the number of immigrants per generation (90% credibility range is presented in the parenthesis), whereas regular numbers are mutation-scaled effective immigration rates. Arrows indicate migration direction.
Table 3.
Genetic polymorphism revealed inside populations of C. narentana and C. illyrica based on cyt b/RAG 1/S7 first intron.
Fig 4.
Bayesian skyline plots based on cyt b sequences.
Changes in effective population size (millions of individuals on a log scale; Y-axes) are depicted over time (in million years; X-axes). Black central lines represent the median values of Ne, while blue lines represent the 95% highest posterior density of the Ne estimates.
Table 4.
Effective population sizes (Ne) and extrapolated census population sizes (N) based on Ne/N = 0.1–0.2.