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Fig 1.

The study design, oral glucose tolerance test, and statistical analysis protocol.

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Fig 1 Expand

Table 1.

Characteristics of subjects at entry into the oral glucose tolerance test.

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Table 1 Expand

Fig 2.

Classification of glucose tolerance using the oral glucose tolerance test.

Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3.

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Fig 2 Expand

Fig 3.

Classification of insulin resistance using the oral glucose tolerance test.

Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3. Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic).

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Fig 3 Expand

Table 2.

Correlations of the fasting plasma levels of 10- and 12-(Z,E)-HODE/LA, RBP4, glycoalbumin, hs-CRP, adiponectin, leptin, and L/A with glucose, insulin, HbA1c, Matsuda Index 3, and HOMA-IR during the OGTT.

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Table 2 Expand

Fig 4.

Correlation of 10- and 12-(Z,E)-HODE fasting plasma levels with (A) RBP4 and (B) glycoalbumin.

Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3. Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). 10- and 12-(Z,E)-HODE, 10- and 12-(Z,E)-hydroxyoctadecadienoic acid; RBP4, retinol binding protein 4.

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Fig 4 Expand

Fig 5.

Predicting the risk of glucose tolerance and insulin resistance.

Matsuda Index 3 is estimated using the 9 biomarkers that are shown in Model 1. Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3. Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic).

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Fig 6.

Selecting biomarkers for practical use.

Matsuda Index 3 is estimated using 10- and 12-(Z,E)-hydroxyoctadecadienoic acid, insulin, and leptin/adiponectin (Model 2) (A), and the risk of glucose tolerance and insulin resistance is predicted using fasting levels of glucose and estimated Matsuda Index 3 (B). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3. Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic).

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Fig 6 Expand