Fig 1.
Visible Light Enhanced Pigment Deposition in human skin explants.
Human skin explants were exposed to visible light 150 J.cm-2 (day 1, 2, 3, 6, 7) and harvested at day 7 for quantification of melanin content.
Fig 2.
DRS results showing the changes of absorption induced by visible light exposures on the skin explants.
A) Pigmentation represented by the area of differential absorption at 620–700 nm. Only the samples of visible light exposure showed absorption changes with statistical significance (p < 0.05). B) Representative spectral changes in absorption between baseline and post-exposure on day 7.
Fig 3.
Relative expression of Tyrosinase gene expression in Visible Light-Exposed Skin Explants.
Human abdominal skin biopsies were exposed to Visible light (150 J.cm-2 at day 1, 2, 3, 6, 7). Graph illustrates the relative expression in explants harvested day 3 and day 7 respectively.
Fig 4.
Tyrosinase enzyme activity at day 3 and day 7 for visible light exposure and sham treatment.
Fig 5.
Illustrates the cross-polarized image of a subject skin phototype V for: a) baseline, no irradiation; b) 300 J.cm-2 day 1; c) 150 J.cm-2 day 2; d) 150 J.cm-2 day 3; e) no irradiation day 4; f) no irradiation day 10.
The dots represent the permanent marker ink used to localized the irradiate site. The white scale bar is from the close up accessory that illustrates the millimeter scale to provide dimensional information. Space in between two consecutive black lines in the white scale represents 1mm distance.
Fig 6.
A) Illustrates the amount of facultative pigment induced after the initial conditioning dose of 300 J.cm-2 and the lower daily irradiation dose of 150 J/cm2 for different skin phototypes. Figure shows that a small daily dose increases or maintains the skin pigmentation induced by the initial conditioning dose. B) Spectral characteristics of the pigment content induced by visible light after multiple exposures. Spectral changes illustrate gradual changes from IPD type of response (day1) to a spectral feature that is similar to native pigment (day 10). For comparison, spectrum for native pigment is inserted to show gradual changes of pigmentation from multiple exposures of visible light to native pigment on day 10.