Fig 1.
A simplified pathway for carotenoid biosynthesis in Synehocystis.
Only enzymes with verified function are included.
Fig 2.
Carotenoid distribution in Synechocystis membranes.
Total carotenoid amount in purified PM1, PM2 and TM (A). Relative amount of different carotenoids in each membrane (B). Data represent means ± standard deviations for carotenoids in membranes from three independent cultures. For results of statistical analysis, please refer to S2 Table.
Fig 3.
Western blots analyses of purified PM1, PM2 and TM from CrtQ-FLAG and CrtO-FLAG strains. Exposure times were 4 and 180 seconds respectively (A). Quantifications of CrtQ (grey) and CrtO (white) in each membrane, based on four independent western blots (B). Data represent means ± standard deviations for four independent quantifications. For results of statistical analysis, please refer to S2 Table.
Fig 4.
Membrane binding analyses of CrtQ-FLAG and CrtO-FLAG.
Western blots of soluble fractions vs. total membranes (5 μg each) isolated from both strains and the effect of different washes on CrtO and CrtQ membrane association. Total membranes were washed with EB8, EB12 or EB8+U and soluble (“Sol.”) and pellet (“Pel.”) fractions were generated by ultracentrifugation. Each lane was loaded with equal volumes of (resuspended) pellet or supernatant fractions. Three independent cultures were tested in this manner. (A). Western blots analysis of anti-FLAG resin eluates from 2% DDM-solubilized total membranes of WT, CrtO-FLAG and CrtQ-FLAG cells, separated on 4–18% Clear Native–PAGE (B). Colour scan of CN-PAGE gel for WT total membrane showing the relative migration of several known complexes.