Fig 1.
Schematic diagram of the MMPs-TRFIA for determinations of HBeAg, Anti-HBs and FT4.
Fig 2.
Calibration curves for MMPs-TRFIA for determinations of HBeAg (A), Anti-HBs (B) and FT4 (C) standards.
Fluorescence intensity and standard deviations were calculated from a set of five measurements.
Fig 3.
Functional detection limits of HBeAg, Anti-HBs and FT4 were the lowest analyte concentration that could be measured with a CV of 15% or less in ten replica measurements.
Table 1.
Comparison of sensitivity and linear range for the determination of HBeAg, Anti-HBs and FT4 by previously reported methods.
Table 2.
Accuracy of MMPs-TRFIA was calculated by measuring seril dilutions of known concentration substances and these results were compared to the quantity's actual value.
Table 3.
Assay precision: intra-assay and inter-assay stability of the MMPs-TRFIA.
Table 4.
Assay specificity: effect of potentially interfering substances on the determination of HBeAg, Anti-HBs and FT4.
Fig 4.
Plot of the results obtained with the proposed method versus those obtained using CLIA for 184 positive HBeAg samples (A), 210 positive anti-HBs samples (B) and 202 FT4 samples(C).
Table 5.
McNemar’s test between the proposed method and CLIA for the test of 257 HBeAg samples.
Table 6.
McNemar’s test between the proposed method and CLIA for the test of 269 anti-HBs samples.