Fig 1.
Electrolyte leakage of two DH lines, Chiifu and Kenshin, subjected to treatment at 45°C.
Leakage is expressed as the ratio (%) of the conductivity of the initial (I) and final (F) solution. Error bars represent SD of six replicates.
Fig 2.
Distribution of genes up- or down-regulated by heat.
A, Distribution of genes up-regulated by heat in Chiifu; B, Distribution of genes up-regulated by heat in Kenshin. Gray number means the stepwise priming up-regulated genes. C, Distribution of heat down-regulated genes in Chiifu; D, Distribution of heat down-regulated genes in Kenshin. Black numbers indicate the genes down-regulated at a single time point or continuously.
Fig 3.
qRT-PCR analysis of several SEGs from either Kenshin or Chiifu.
Relative expression was normalized against BrActin. Two biological repeats were performed, and error bars represent SD. HS, heat shock.
Table 1.
GO annotation of intrinsically expressed genes.
One gene can be associated with two or more GO terms. NA, no Arabidopsis homologs.
Fig 4.
Heat map of genes induced/repressed by HS treatment.
Table 2.
Summary of transcription factor genes represented on the Br135K microarray.
Fig 5.
qRT-PCR analysis of transcription factor genes that were up-regulated by HS treatment.
Relative expression was normalized to BrActin and is presented relative to 0 h expression levels. Two biological repeats were performed, and error bars represent SD. HS, heat shock.
Fig 6.
Expression patterns of marker genes for thermotolerance in Chiifu and Kenshin.
PI value indicates probe intensity (see also S20 Table). A, Expression patterns of basal thermotolerance marker genes (BT) and short-term acquired thermotolerance (SAT) in Chiifu and Kenshin. B, Expression patterns of long-term acquired thermotolerance (LAT) marker genes in Chiifu and Kenshin. C, Expression patterns of thermotolerance to moderately high temperatures (TMHT) marker genes in Chiifu and Kenshin.
Fig 7.
Examples of membrane leakage-related (MLR) genes.
A, Four genes: two up-regulated genes, Bra032994 (NA) and Bra025083 (cytochrome P450; CYP707A3), and two down-regulated genes, Bra006613 (FAD/NAD(P)-binding oxidoreductase) and Bra016265 (protein kinase superfamily protein). B, Three B. rapa genes for CYP707A3. C, qRT-PCR of three B. rapa cytochrome P450 genes (abscisic acid 8’-hydroxylase). Relative expression was normalized to BrActin and is presented relative to 0 h expression levels. Two biological repeats were performed and error bars represent SD. HS, heat shock.
Fig 8.
Heat map of orphan genes induced by HS treatment.
Fig 9.
qRT-PCR verifications of the expression of several orphan genes.
Relative expression was normalized to BrActin and is presented relative to 0 h expression levels. Two biological repeats were performed, and error bars represent SD. HS, heat shock.
Fig 10.
Short Time-series Expression Miner (STEM) clusters of expression profiles with HSR genes in Chiifu.
The number of profiles in each cluster is at the top left corner of each STEM. Profiles are ordered based on the p-value significance of the number (at bottom-left corner) of assigned versus expected genes. Colored frame denotes significant profiles (P-value≤0.01). Each graph displays the mean expression pattern (black lines) of the profile genes.