Fig 1.
A) Histograms of values obtained for gels with varying collagen type III concentration.
The bars represent experimentally acquired data. The red line is the bimodal Gaussian fit to the data. B) Standard curve for collagen type III concentration. The known concentration of collagen concentration is plotted against the ratio of the area under the collagen type III peak ~0.8 to the total area under the Gaussian fit.
Fig 2.
Masson’s Trichrome staining of representative sections subcutaneously injected.
(A) Representative sections stained with Masson’s Trichrome are shown for the various polystyrene particles injected into mice. (Magnification 20×, images are 540 × 360 μm2) (B) Quantification of capsule thickness. Error bars represent the standard deviation.
Fig 3.
A) Representative SHG images of the fibrotic capsule in tissue section with different subcutaneously implanted polystyrene particles. SHG intensity is in blue pseudocolor. The scale bar represents 20μm. B) SHG polarimetry profiles for tissue sections with on different latex beads. The y-axis corresponds to SHG intensity in arbitrary units and the azimuthal angles correspond to polarization angles.
Fig 4.
Shape descriptors of fibrotic capsules surrounding subcutaneously implanted polystyrene particles.
For D = 1 collagen orientation is uniaxial, while for collagen fibers with D = 0 orientation is completely random. All experiments were done in quadruplicate. Results are expressed as the mean value ± standard deviation. (*) indicates p<0.05.
Fig 5.
A) Histograms of values obtained for tissue sections of subcutaneously implanted aldehyde/sulfate, amidine, amino and dimethylamino polystyrene particles. The bars represent experimentally acquired data. The red line is the bimodal Gaussian fit to the data. B) Collagen type III content of fibrotic capsules surrounding subcutaneously implanted polystyrene particles.
Fig 6.
Representative immunofluorescence images of mouse tissues subcutaneously injected with various polystyrene particles.
Green is collagen type I, red is collagen type III, and blue is DAPI. The scale bar represents 100 μm.
Fig 7.
Heat map comparison of fibrotic capsule thickness, % of collagen type III, and the D shape factor.