Table 1.
Growth of Methylobacterium-inoculated rice.
Fig 1.
MSP dendrogram based on WC-MS analysis of the isolates from leaves of inoculated rice seed.
The isolates were named with the treated strain name and isolate numbers. The inoculated strains are colored in red. The representatives selected from each cluster are colored in blue with the closest type strain name and percentage identity of 16S RNA gene in parentheses. Isolate 22A-9 was pink-pigmented fungus and was identified by ITS region sequencing, as described previously [19].
Fig 2.
Identification and composition of Methylobacterium species isolated from rice plants subjected to Methylobacterium inoculation.
Total number of Methylobacterium isolates is shown in parentheses. Identification was performed by WC-MS and 16S RNA sequencing, and details are shown in Fig 1.
Table 2.
Growth of Methylobacterium-inoculated barley.
Fig 3.
MSP dendrogram based on WC-MS analysis of the isolates from barley of different cultivars.
The representatives are shown in blue with the closest species name and percentage identity of the 16S RNA gene in parentheses. Strains shown in gray were not pink-pigmented.
Table 3.
Isolates’ genotypes and characteristics of barley.
Fig 4.
Maximum-likelihood phylogenetic tree of Methylobacterium isolates and related taxa, based on 16S rRNA gene sequences.
Isolates from rice are colored in green and those from barley are in blue. Numbers in parentheses indicate isolates belonging to the same species, estimated by WC-MS analysis. For isolates from rice, the inoculation effect is not taken into account in the figure. M aquaticum strain 22A is taken as a representative strain for the M. platani/aquaticum cluster shown in Fig 1. Bootstrap percentages based on 1000 replicates are shown if greater than 80%. Microvirga flocculans TFB (AB098515) was used as an outgroup. Bar, 0.1 changes per nucleotide position.