Fig 1.
Frequency distribution curve for nine agronomic traits including 1000 grain weight in 207 Indian wheat genotypes used in association mapping study.
Table 1.
Descriptive statistics for 1000 grain weight and other agronomic traits.
Fig 2.
Representative gel picture of PCR amplification of promoter region of TaGW2-6A gene in 15 Indian wheat genotypes.
Table 2.
Summary of four SNPs (identified by sequence alignment) with their position, variation, frequency and count.
Table 3.
Summary of marker-trait association using single SNP for 1000 grain weight.
Significantly associated SNP is marked with *.
Table 4.
Marker-trait association of SNP-494 with 1000 grain weight and other eight agronomic traits.
Fig 3.
Five haplotypes with single nucleotide polymorphisms (SNPs) in the promoter regions of TaGW2-6A.
Frequency of each haplotype is given in parentheses. SNPs are highlighted with yellow (G allele) and red (A allele) colours. CGCG motif is shown in a box. Significant difference of mean TGW (g) is represented by bars. Haplotypes covered by a single bar represent no significant difference for TGW and vice-versa.
Table 5.
Analysis of variance for TGW using five different haplotypes.
Fig 4.
Bar diagrams showing (A) relative expression level of TaGW2-6A in immature seeds at 15dpf.
Actin gene was used as the endogenous control and variety HI 1500 used as reference; (B) TGW of varieties having SNP-494_A on the left panel and those with SNP-494_G in the right panel.
Fig 5.
Plot showing significant regression of (A) TaGW2-6A expression level and thousand grain weight (TGW) in genotypes with two different alleles (A and G) of SNP-494 identified in promoter region, and (B) TGW with relative expression level in same genotypes.
* and ** indicate significance at 0.05 and 0.01 levels, respectively.
Fig 6.
Validation of CAPS in genotypes with causal SNP_G and SNP_A on 2% agarose gel.
G and A represent undigested PCR products of genotypes with SNP_G and SNP_A; G1–G4 and A1–A4 are genotypes with SNP_G and SNP_A after digestion with FauI, M = marker.
Fig 7.
A hypothetical pathway of CGCG mediated regulation of TaGW2-6A.