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Table 1.

Subject Demographics of Duchenne muscular dystrophy (DMD) and age matched control (Ctrl) boys.

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Fig 1.

T1 weighted images of lower leg muscles.

Representative T1 weighted 3-D gradient echo images of A) control, and B) DMD subject. Outline of regions of interest (A) showing different muscles namely TA, tibialis anterior; EDL, extensor digitorum longus; Per, peroneal; MG, medial gastrocnemius; SOL, soleus; TP, tibilais posterior; FDL, flexor digitorum longus. (B) Showing areas of fatty tissue infiltration in leg muscles.

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Fig 1 Expand

Fig 2.

Contractile cross sectional area (C-CSA), non-contractile cross sectional area (NC-CSA), and non-contractile content (NCC) of all the lower leg muscles of boys with DMD and healthy control subjects.

Significant difference was found in NC-CSA and of peroneal (Per), medial gastrocnemius (MG), and soleus (SOL) (denoted by **, p<0.05). No differences were observed in C-CSA of DMD and Ctrl leg muscles when all age groups were combined. Data is presented as mean (SD).

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Fig 3.

Percent non-contractile content (NCC) in leg muscles of Ctrl and DMD in different age groups.

NCC of leg muscles of DMD at the age of >10 years was significantly different from 5–7.9 and 8–9.9 years age groups (denoted by #, p<0.05). NCC of TA, EDL, Per, MG, and SOL of DMD were significantly different than Ctrl in > 10 year age group (denoted by *, p<0.05). Additionally, only MG showed significant differences between DMD and Ctrl group at the age of 8–9.9 year. Data is presented as mean (SD).

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Fig 3 Expand

Fig 4.

Comparison of C-CSA and NC-CSA among different age groups in DMD and control groups.

Red and blue color represents N-CSA and C-CSA respectively. Symbol colors are accordant with the bar colors (red; N-CSA, blue; C-CSA). * Represents significant differences between DMD and age matched Ctrl group (p<0.05); # represents significant differences across the age groups (p<0.05).

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Fig 4 Expand

Table 2.

Comparison of timed functional tests between control (Ctrl) and subjects with Duchenne muscular dystrophy (DMD).

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Fig 5.

Relationship between NCC of different leg muscles and 30 feet walk (sec).

There was a significant relationship between increase in NCC of all leg muscles and time taken to cover 30 feet distance (rs; 0.65–0.80, p<0.0001).

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Fig 5 Expand

Fig 6.

Relationship between NCC of different leg muscles and Brooke score.

There was a significant relationship between NCC of all leg muscles and Brooke score (rs; 0.64–0.84, p<0.0001).

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Fig 6 Expand

Fig 7.

Comparison of dorsiflexors (DF) and plantarflexors (PF) peak torque (A, B) and specific torque in boys with DMD and healthy Ctrl across different age groups.

* represents significant differences between DMD and Ctrl within age groups (p<0.05), # represents significant differences across the age groups (p<0.05). Data is presented as mean (SD).

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Fig 7 Expand