Fig 1.
Anti-HEV antibody titres in positive canine serum samples.
Positive canine serum samples were prepared in dilutions of 1:100, 1:200, 1:400, 1:800; 1:1600 and 1:3200 and used in an ELISA assay. The corrected OD450 was obtained by subtracting the background signal from the VLP coated well OD450 value. The positive threshold was determined by calculating the mean OD450 of buffer coated wells with the highest serum dilution, plus 3 standard deviations.
Fig 2.
Western blot analysis of serum sample reactivity with HEV, vesivirus 2117 and human norovirus G1.1 VLPs.
Three types of VLP were separated by SDS-PAGE. One gel was stained with Coomassie Blue to identify VLP protein at the expected molecular weight. Additional gels were used for western blotting with canine serum samples positive by ELISA for HEV (samples A and B). A pig serum sample (kind gift from S. Emerson) and a human serum sample known to be positive for anti-HEV antibody were used as a positive control for the HEV VLPs. Canine sample C, previously confirmed positive for anti-vesivirus antibody by ELISA, was used as a positive control for the vesivirus VLPs. Canine sample D was used as a negative control for all VLPs.