Table 1.
Primer sequences used for PCR.
Fig 1.
Confirmation of the C. Cayetanensis mt genome sequence.
A) Overlapping PCR fragments used to confirm the C. cayetanenesis mt genome sequence and concatemeric structure. PCR fragments are shown with dotted lines. Forward sequence reads are shown in blue or orange and reverse reads are shown in red or light blue. Terminal grey bands indicate identical sequence regions. B) A 659 bp contig constructed using overlapping junction PCR fragment sequences (see Table 2) is represented in gray. Diagonal red bars represent portions of the junction PCR product mapping to the ends of the complete C. cayetanensis mt genome. C) WGS reads spanning the junction region in the initial mt assembly. The vertical red lines mark the tail:head junction.
Table 2.
PCR primer sets and amplicons.
Fig 2.
Gene arrangement of the C. cayetanensis mt genome.
Predicted genes on the Cyclospora mitochondrial genomes were derived based on ClustalW alignment with the Eimeria gallopavonis (KJ608417). Orange indicates coding genes, blue indicates fragments of LSU rRNA, and yellow indicates fragments of SSU rRNA. Transcriptional direction is indicated by arrowed end.
Fig 3.
Phylogenetic relationships among Eimeria species and C. cayetanensis based on mitochondrial genome sequences.
NCBI accession numbers are included. The evolutionary relationship between the Eimeria (13), Cyclospora(1) and P.falciparum (1) mitochondrial genomes was inferred using the Maximum Likelihood method with 500 replications for bootstrap analysis. The confidence value for clustering is given as percentage above the branches. The scale bar points to the number of substitutions per site. After analysis, the outgroup branch was removed for clarity. Default parameters in MEGA6 were retained for phylogenetic analysis and tree building.