Fig 1.
A maximum likelihood phylogenetic tree of odorant binding proteins (OBPs) from moths.
Includes sequences from Epiphyas postvittana (Epos), Bombyx mori (Bmor), Cydia pomonella (Cpom), Chilo suppressalis (Csup), Dendrolimus houi (Dhou), Dendrolimus kikuchii (Dkik), Helicoverpa armigera (Harm), Heliothis virescens (Hvir), Lymantria dispar (Ldis), Mamastra brassicae (Mbra), Manduca sexta (Msex), Plutella xylostella (Pxyl) and Spodoptera exigua (Sexi). OBPs identified from E. postvittana are in bold. Node support was assessed using bootstrap replicates of 1000 and values greater than 50% are shown. Sub-groups including the PBPs, GOBPs, minus-Cs and plus-Cs are marked.
Fig 2.
A maximum likelihood phylogenetic tree of odorant receptors (ORs) from moths.
Includes sequences from Epiphyas postvittana (Epos), Cydia pomonella (Cpom), Heliothis virescens (Hvir) and Bombyx mori (Bmor), and is rooted with the OR co-receptor, Orco. The lepidopteran ‘pheromone receptor’ clade is shaded. E. postvittana ORs are shown in bold font with male-biased E. postvittana ORs colored blue and female-biased E. postvittana ORs colored pink. Node support was assessed using bootstrap replicates of 1000 and values greater than 50% are shown.
Fig 3.
A maximum likelihood phylogenetic tree of antennal carboxylesterases (CXEs) from moths.
Includes sequences from Epiphyas postvittana (Epos), Bombyx mori (Bm), Antheraea polyphemus (Apol), Mamestra brassicae (Mbra), Spodoptera littoralis (Slitt), Sesamia nonagrioides (Snon), Spodoptera litura (Slitu) and Spodoptera exigua (Sexi). CXEs identified from E. postvittana are in bold and those predicted to be secreted are indicated with an asterisk. The predicted pheromone degrading enzyme is shown in red. Node support was assessed using bootstrap replicates of 1000 and values greater than 50% are shown.
Table 1.
Transcript counts in antennal tissue from male and female Epiphyas postvittana as determined by RNAseq.
Table 2.
Odorant receptor (OR) mRNA expression in antennal and body tissue of female and male Epiphyas postvittana as determined by quantitative RT-PCR and OR transcript counts in antennal tissue as determined by RNAseq.
Fig 4.
Male-biased (A) and female-biased (B) antennal odorant receptor mRNA expression in Epiphyas postvittana.
Comparison of OR mRNA expression in male (♂) and female (♀) antennae as determined by quantitative real-time PCR (qPCR; left panel) and RNASeq (right panel). For qPCR data, bars represent mean (± SEM) of OR mRNA expression relative to reference genes from three biological replicates. For RNAseq data, bars represent transcript counts from a single transcriptome made from male or female antennal mRNA.