Fig 1.
Consecutive administration of two different thymidine analogs, namely CIdU (in red) and IdU (in green), to mice allows the detection of serially replicating cells (CIdU-IdU-co-labeled cells, in orange). (A): According to the experimental methodology developed by Teta et al. [3], if the prostate epithelium is maintained by transit-amplifying (TA) cells that undergo consecutive cycles of cell division, a significant number of proliferating cells would be co-labeled by both thymidine analogs. In that case, the observed fraction of co-labeled cells should be greater than predicted fraction of co-labeled cells by the stochastic model. (B): In contrast, if the epithelium is maintained by cell division that occurs stochastically, the majority of replicating cells should be labeled with either one or the other thymidine analog. In this situation, the observed fraction of co-labeled cells should similar to the fraction of co-labeled cells predicted by the stochastic model. (C): The fraction of co-labeled cells predicted by the stochastic model is calculated by multiplying the fraction of CIdU-labeled cells by the fraction of IdU-labeled cells. This figure has been adapted from Humphreys et al. [8].
Fig 2.
Renewal of the adult prostate epithelium does not depend on rapidly serially proliferating progenitor/TA cells.
Detection of rapidly proliferating progenitors was performed on 7-week-old mice sequentially treated with CIdU and IdU for 1 day each. Mice were sacrificed immediately after the end of IdU administration. (A): Representative images of double immunofluorescence staining for CIdU and IdU in a section of small intestine demonstrating the presence of double-labeled progenitor cells in the intestinal crypt (inset). (B): Graphic representation of the percentages of intestinal epithelial cells labeled with CIdU, IdU, or CIdU/IdU. The predicted stochastic fraction is also shown. Data represent the means ± SD for three mice per group. (C, D): Representative images of triple immunofluorescence staining for CIdU, IdU and Krt14 performed on sections of the distal/intermediate region of ducts (C) and the proximal region of ducts (D) of the dorsal prostate showing that the majority of cells are single labeled (inset). Yellow arrowheads indicate CIdU-IdU-co-labeled cells. (E): Graphic representation of the percentages of epithelial prostate cells (both basal and luminal) labeled with CIdU, IdU, or CIdU/IdU (DL). The predicted stochastic fraction is also shown. Data represent the means ± SD for three mice per group. n indicates the average number of nuclei counted per mouse.* indicates p<0.05.
Fig 3.
Renewal of the adult prostate epithelium does not depend on slowly serially proliferating progenitor/TA cells.
Representative images of triple immunofluorescence staining for CIdU, IdU and Krt14 on sections of the distal/intermediate region (A, C, E, G) and the proximal region of ducts (B, D, F, H) of the dorsal prostate of 7-week-old mice treated for either 1 week with CIdU followed by 1 week of IdU (A, B), or 1 week with CIdU followed by 1 month of IdU (C, D), or for 1 month of CIdU followed by 3 months of IdU (E, F), or for 1 month of CIdU followed by 9 months of IdU (G, H). In all experiments, mice were sacrificed immediately after the end of IdU administration. Yellow arrowheads indicate double-labeled cells while insets show that the majority of the cells are single labeled. (I): Graphic representation of the percentages of epithelial prostate cells (both basal and luminal) labeled with CIdU, IdU, or CIdU/IdU (DL) for the indicated treatment groups. The predicted stochastic fraction is also shown. Data represent the means ± SD for three mice per group. n indicates the average number of nuclei counted per mouse.
Fig 4.
Androgen mediated regeneration of the prostate epithelium after castration does not depend on rapidly serially proliferating progenitor/TA cells.
Representative images of triple immunofluorescence staining for CIdU, IdU and Krt14 on sections of the distal/intermediate (A, C) and the proximal (B, D) regions of ducts from the dorsal prostate of castrated mice sequentially treated with CIdU and IdU (1 day each) at day 2 (A, B) or day 3 (C, D) after androgen supplementation. In all experiments, mice were sacrificed immediately after the end of IdU administration. Yellow arrowheads indicate double-labeled cells while insets show that the majority of the cells are single labeled. (E): Graphic representation of the percentages of epithelial prostate cells (both basal and luminal) labeled with CIdU, IdU, or CIdU/IdU (DL) for the indicated treatment groups. 2dR and 3dR indicate mice that were treated with the thymidine analogs at day 2 or day 3 after androgen supplementation, respectively. The predicted stochastic fraction is also shown. Data represent the means ± SD for three mice per group. n indicates the average number of nuclei counted per mouse. * indicates p<0.05.