Fig 1.
Network of fatty acid biosynthesis in oleaginous eukaryotic microorganisms.
HK, hexokinase; PFK, phosphofructokinase; G6PDH, glucose-6-phosphate dehydrogenase; 6PGDH, 6-phosphogluconate dehydrogenase; CS, citrate synthase; ICDH, isocitrate dehydrogenase; ACL, ATP:citrate lyase; ME, malic enzyme; FAS, fatty acid synthase.
Fig 2.
Cell growth and lipid accumulation of M. circinelloides WJ11 (◆) and CBS 277.49 (◇).
(a) Cell dry weight (CDW), (b) ammonium and (c) glucose concentration in culture medium, and (d) total fatty acids (TFAs) content (w/w, TFAs/CDW) of strain WJ11 and CBS 277.49 grown in the modified K & R medium in 2L fermenter were measured. Values were mean of three biological replicates. Error bars represent the standard error of the mean. * P < 0.05 significantly different between the two strains.
Table 1.
Fatty acid composition (%, w/w of total fatty acids) of M. circinelloides WJ11 and CBS 277.49 during the fermentation.
Fig 3.
Activity of key enzymes related to lipid accumulation in M. circinelloides WJ11 (●) and CBS 277.49 (○).
Enzymes measured were (a) ACL, (b) ME, (c) G6PDH, (d) 6PGDH, (e) AMPD, (f) NAD+:ICDH, (g) NADP+:ICDH, (h) CS, (i) HK, (j) PFK, (k) FAS. Values are mean of three biological replicates. Error bars represent the standard error of the mean. * P < 0.05 significantly different between the two strains.
Fig 4.
Effect of AMP concentration on NAD+:ICDH activity in M. circinelloides WJ11 (a) and CBS 277.49 (b).
The activity of NAD+:ICDH of the cell extract at 24 h grown in the modified K & R medium in 2L fermenter was measured, in vitro, in the presence and absence of AMP at different isocitrate concentrations. Isocitrate concentrations: 0.1 mM (○); 0.5 mM (●); 1 mM (◆); 2 mM (■); 5 mM (□). Values are mean of three biological replicates. Error bars represent the standard error of the mean.