Fig 1.
Representative images of hematoxylin-eosin staining of in vitro wounds.
(A) Section of re-epithelialized wound bed with incorporated microcarriers; (B) section of re-epithelialization of control wound. (C and D) Magnifications of wound edge of (A) and (B). Scale bar A-D = 500 μm. (E) Section of wound cultured submerged in culture medium for 14 days and air-lifted for seven days with microcarriers; (F) Wound edge of air-lifted control wound. Scale bar E-F = 200 μm.
Fig 2.
Measurement of neoepithelial thickness.
(A) Average thickness of neoepidermis in wounds cultured with CultiSpher-S microcarriers (n = 15) compared to control wounds (n = 11; asterisk indicates significance, p < 0.05). (B) Immunohistological staining with antibodies against cytokeratin (red) of wound cultured with microcarriers; arrow indicates neoepidermis, arrowheads indicate wound edges. (C) Immunohistological staining with antibodies against pancytokeratin of control wound; arrow indicates neoepidermis, arrowheads indicate wound edges. Scale bar = 1000 μm for (B) and (C).
Fig 3.
(A) Immunohistological staining against keratin 10 (red) and keratin 5 (green) in neoepidermis with incorporated microcarriers and compared to (B) normal control skin. (C) Immunohistological staining against pancytokeratin (red) showing microcarrier populated by keratinocytes. (D) Immunohistological staining against laminin 5 (green) in neoepidermis with microcarriers, (E) unwounded skin, and (F) neoepidermis of control wound. Nuclear staining with 4, 6-diamidino-2-phenylindole (DAPI). All scale bars = 100 μm.