Table 1.
Details of experimental groups and treatments.
Fig 1.
Photographs showing typical clinical signs in Trypanosoma evansi infection in rabbits.
A. Lacrimation B. Corneal opacity C. Swelling of external genitalia. a. T. evansi infected rabbit b. CpG C treated rabbit challenged with T. evansi
Fig 2.
Effects of CpG-ODN C treatment in Trypanosoma evansi infected and uninfected rabbits.
The rabbits of group I were infected with 1x105 T. evansi parasites/animal. The group II rabbits were treated with CpG C formulated with 10% oil-in-water emulsion and then challenged with 1x105 T. evansi parasites/animal. The rabbits of group III, IV and V were inoculated with formulated CpG C, CpG C alone and PBS as negative control, respectively. Effects of CpG-ODN treatment and/or T. evansi infection. A. Rectal temperature (°F). B. The number of rabbits showing parasitemia in wet blood film during the course of the disease in both the infected groups were determined and expressed in percentage. Figure B depicts the percent of rabbits of groups I and II showing parasitemia on different days. C. Parasitemic scores on different days in groups I and II. D. Percent decrease in haemoglobin level in groups infected with T. evansi.
Table 2.
Effects of CpG ODN inoculation and/ or Trypanosoma evansi infection on mean haemoglobin values (g/dl) in different groups of rabbits.
Table 3.
Effects of CpG ODN inoculation and/ or Trypanosoma evansi infection on mean blood glucose values (g/dl) in different groups of rabbits.
Fig 3.
Effects of CpG ODN inoculation and/ or T. evansi infection.
A. Immunoglobulin G values (g/l) in different groups of rabbits. The anti-rabbit IgG antibodies formed insoluble complexes when mixed with samples containing purified rabbit IgG. IgG concentration in the sample was quantified by comparing with a calibrator of known IgG concentration. B. Relative percent positivity for T. evansi specific Immunoglobulin G. T. evansi specific IgG levels were determined by ELISA and expressed as relative percent positivity with respect to known T. evansipositive serum sample.
Fig 4.
Histopathological changes in liver and spleen of Trypanosoma evansi infected and uninfected rabbits in response to CpG-ODN treatment.
A. Liver tissue of T. evansi infected (positive control) group I, extensive necrosis and loss of normal hepatic architecture is shown by double arrow (H&E 400X). B. Liver tissue of group II (CpG C treated and challenged with T. evansi), decreased severity of necrosis (black colored arrow) (H&E 400X). C. Liver tissue of group III (treated with formulated CpG alone),mononuclear cell infiltration in portal triad (black colored arrow) (H&E 400X). D. Spleen T. evansi infected (positive control) group I, secondary follicles (white block arrows), haemorrhages and edematous fluid(H&E 100X). E. Spleen of group II CpG C treated and challenged with T. evansi. Secondary follicles comparatively more in number (white block arrows) (H&E 100X)