Fig 1.
Schematic representation of the procedure adopted for preparing the explants.
a) The multiple shoots induced from EC seedling; b) the stem segment removed from shoot cluster; c) the removal of axillary bud and leaflet from the stem (arrows indicate surgical excision points); d) a stem segment with one node (SN) ready for adventitious buds induction; e) a shoot with terminal bud explant (TS) ready for elongation and root induction; f) the basal section of the multiple shoots (BMS) ready for propagation of EC seedlings of C. camphora.
Fig 2.
Cotyledonary embryo-cultured seedlings propagation of C. camphora.
a) Germination induction for cotyledonary embryo of C. camphora, bar: 1.0 cm; b) EC seedlings transferred to the light culture, bar: 1.0 cm; c) multiple shoots sprouting from the basal section of seedling, bar: 1.0 cm; d) EC seedling line with high proliferation, bar: 1.0 cm; e) the basal section of the multiple shoots (BMS explant) from EC seedling, bar: 0.3 cm; f) the shoots proliferation of BMS explant after 2 weeks culture, bar: 1.0 cm.
Fig 3.
Plantlet regeneration from stem segment with nodule of embryo-cultured seedlings of C. camphora.
a) a stem segment with one node (SN explant) for adventitious buds induction (arrow indicates the nodal region without axillary bud) bar: 0.1 cm; b) adventitious buds induced from SN explants, bar: 1.5 cm; c) direct organogenesis in SN explant, bar: 0.3 cm; d) a regenerated shoot with terminal bud from SN explants for elongation and root induction (arrow indicates excision points of lateral buds), bar: 0.3 cm; e) regenerated plantlet in vitro, bar: 1.0 cm; f) hardened plants, bar: 1.5 cm.
Table 1.
Effect of different media compositions on adventitious buds induction from SN explants of C. camphora.
Table 2.
Effect of explants donor genotype on adventitious buds induction from SN explants of C. camphora.
Table 3.
Effect of different treatments on the root induction from regenerated shoot of C. camphora.
Fig 4.
Schematic representation of the plantlet regeneration protocol for C. camphora.