Fig 1.
Epiphyllous ovule leaves and normal leaves from G. biloba var. epiphylla.
(A) Abnormal ovuliferous structure with partly epiphyllous ovules leaves and normal leaves in the early stage of partly epiphyllous ovule germination (in mid-April). (B) Close-up of the epiphyllous ovules leaves (in mid-April).
Table 1.
Summary of small RNA sequencing statistics.
Table 2.
Summary of the clean small RNAs in the epiphyllous ovule leaves and normal leaves libraries.
Table 3.
Non-coding RNAs among the small RNAs in the epiphyllous ovule leaves and normal leaves libraries.
Fig 2.
Length distribution of small RNAs from the epiphyllous ovule leaves (EL) and normal leaves (NL).
Fig 3.
Numbers of members identified in the 23 conserved miRNA families.
Fig 4.
Differentially expressed miRNAs in epiphyllous ovule leaves (EL) compared with normal leaves (NL).
Only miRNAs that identified in both datasets with fold change higher than 1.5 are shown.
Fig 5.
GO annotation of the targets genes of the differentially expressed miRNAs.
Fig 6.
Differentially expressed miRNAs between epiphyllous ovule leaves (EL) and normal leaves (NL) by RT-qPCR.
(A) miRNAs with expression levels up-regulated in EL compared with their expression levels in NL. (B) miRNAs with expression levels down-regulated in EL compared with their expression levels in NL. The expression levels of the miRNAs were normalized to that of 18S rRNA. Bars indicated the standard error of means.
Fig 7.
Expression patterns of the ten predicted target genes of the selected miRNAs.
The expression levels of the target genes were normalized to that of GAPDH. Bars indicated the standard error of means.