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Table 1.

List of the primers and probes used in this study.

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Table 2.

ISSau2-positive S. aureus strains and isolates evaluated in this study.

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Fig 1.

Relationship among the ISSau2 containing S. aureus strains and isolates.

The phylogenetic tree of ISSau2 gene was constructed by the MEGA software (version 5.2) using default parameters. Numbers at nodes are levels of bootstrap support (percentages) based on a neighbor-joining analysis of 1,000 resampled datasets using the Maximum Composite Likelihood method. Four groups (A, B, C and D) of 27 S. aureus strains and 10 isolates were indicated. The strains or isolates in a same branch shared identical ISSau2 sequences.

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Fig 2.

A schematic map of ISSau2 and its adjacent sequence in strain LGA251 and strain ST398.

The ISSau2 and its adjacent sequences were designated as different colors of shapes. The blue “A” represents a hypothetical protein; the brown “B” is a putative membrane protein; the red “C” stands for a putative short chain dehydrogenase; the “U” represents an unknown protein. The length of each region is also indicated.

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