Table 1.
Clinical and demographic characteristics of studied subjects with chronic periodontitis (n = 34).
Fig 1.
Inter-subject variability of subgingival communities in periodontitis and relationship of microbial profiles with demographic, medical and clinical characteristics.
Graph shows a heatmap of the transformed relative abundances for the top 300 OTUs found in the subgingival microbial communities of subjects with periodontitis. Subjects are shown in rows, while OTUs appear in columns. Color scale for heatmap appears in the top left with the most abundant OTUs in yellow and the least abundant in blue. Subject raws are organized according to results of unsupervised hierarchical clustering (complete linkage) of microbial communities based on OTU relative abundances. Dendogram on the left shows a tendency for 2 clusters to form (A and B). Color bars in the right depict the demographic, medical and clinical characteristics for each subject. Color scales for metadata appear on the right side.
Table 2.
Comparison of mean clinical periodontal parameters between subjects in periodontitis clusters A and B (unsupervised hierarchical).
Fig 2.
Signature OTUs that define periodontitis clusters A and B (hierarchical).
Graph shows OTUs with a statistically significant difference in relative abundance between hierarchical clusters A and B. Bars represent the median and range of relative abundances for each OTU. OTUs increased in Cluster A appear in the top, while OTUs increased in cluster B appear in the bottom. OTUs were labeled according to their association with health or periodontitis considering data from Abusleme et al. [6], Perez-Chaparro et al. [31] and a comparison of our periodontitis samples with HMP healthy subjects (S4 Fig). Taxa were classified as P (periodontitis), H (health) and C (core species), according to at least one study. If a disagreement existed between studies, taxa were labeled under more than one category.
Table 3.
Spearman rank order correlation tests between demographic and clinical characteristics of subjects with periodontitis and the total relative abundance of signature OTUs for clusters A and B (hierarchical).