Fig 1.
General study and meta-analysis scheme.
The workflow is represented by boxes and arrows.
Table 1.
The characteristics of the datasets used for meta-analysis.
Fig 2.
Pathway enrichment of the grade-specific let-7 family targets.
Validated targets of the let-7 miRNAs and KEGG pathway results were used to construct the heatmap. The intensity of color represents the adjusted p value (p≤0.0091).
Table 2.
Top 20 meta-miRNAs and their ranking values.
Fig 3.
Boxplot of let-7 family expression levels.
A consistent decrease in the expression levels of let-7 family members is observed from grade 1 to grade 3 tumors (n = 21).
Fig 4.
In silico analysis of the common genes between validated meta-miRNA targets and meta-mRNAs.
(A) The intersection of meta-mRNAs and let-7-target genes. 116 of the meta-miRNA targets are in common with the meta-mRNAs. (B) Heatmap of the expression of common genes between grade-specific let 7 targets and mRNAs for the GSE22219 dataset. Hierarchical clustering of common genes and grade 1 and grade 3 tumor samples was performed by using average linkage as the clustering method. Red and green colors represent upregulation and downregulation, respectively (p≤0.05). (C) The interaction network between grade predictive let-7 family members and their potential target genes validated by in silico analysis. Target genes are represented by yellow and circular nodes while miRNAs are represented by squares.