Fig 1.
The Cooling-reheating protocol design.
Fig 2.
Segmentation of cervical-supraclavicular and subcutaneous adipose tissue.
A coronal (a) and axial (b) fat fraction (FF) map showing the manually delineated crude cervical-supraclavicular (considered as suspected brown adipose tissue, denoted sBAT) volume of interest (VOI) with a red contour, the final segmented sBAT VOI in green and the final segmented posterior subcutaneous adipose tissue (SAT) VOI in blue. Segmentation of the sBAT and SAT VOIs was accomplished by exclusion criteria on FF, R2* and by erosion.
Fig 3.
Changes in fat fraction and R2* of cervical-supraclavicular and subcutaneous adipose tissue during the Cooling-reheating protocol.
Plots illustrating changes in (a) fat fraction (FF) and (b) R2* of cervical-supraclavicular adipose tissue (considered as suspected brown adipose tissue, denoted sBAT) and subcutaneous adipose tissue (SAT). The fat fraction changes are expressed in percentage points (pp). The time points correspond to the Baseline MRI (t = 0), the Cold MRI (t = 3h) and the Reheated MRI (t = 3.5h). Values plotted represent group means (of individual volume of interest (VOI) means) and standard error of the group means. *p<0.05; **p<0.01; (ns) non-significant.
Fig 4.
Differences in fat fraction and R2* between cervical-supraclavicular and subcutaneous adipose tissue at baseline.
Measurements of (a) fat fraction (FF) and (b) R2* in cervical-supraclavicular adipose tissue (considered as suspected brown adipose tissue, denoted sBAT) and subcutaneous adipose tissue (SAT) in individual subjects from the Baseline MRI. Values plotted correspond to volume of interest means.
Table 1.
Summary of fat fraction (FF) and R2* measurements.
Table 2.
Changes in fat fraction (FF) and R2*.