Table 1.
Quantitative real-time PCR Primers for DNA methytransferases and β-actin.
Table 2.
DEHP induced F1-F4 incidence of cryptorchidism.
Table 3.
DEHP induced male rat reproductive function damage.
Table 4.
DEHP influence male rat’s weight and AGD.
Table 5.
DEHP influence male rat’s testis and epididymis.
Fig 1.
Control group and DEHP experimental group offspring (F1-F4) male rat’s testicular histology expression.
A:F1;B:F2;C:F3;D:F4 E: Control (200×).
Fig 2.
Control group and DEHP experimental group offspring (F1-F4) male rat’s epididymis histology expression.
Normal control group of testicular seminiferous tubule epithelial developed normally, we can observe each stage sperm cells and a large number of sperm cells in the epididymis pipe. DEHP experimental F1 generation observed seminiferous tubule epithelial obvious atrophy, less sperm cells, pathologic hyperplasia of interstitial cells, number of sperm in the epididymis lumen scarce or lack, luminal epithelium cell falls off. F2 generation of rat testis partial seminiferous tubule epithelium atrophy, sperm cells less; F3 and F4 generation were tend to be normal, sperm cells grading well. A:F1;B:F2;C:F3;D:F4 E: Control (200×).
Table 6.
DEHP influence male rat’s sperm counts.
Table 7.
DEHP influence male rat’s semen quality (aberration rate).
Fig 3.
F1-F4 semen quality comparison.
* compared with control group, P<0.05.
Fig 4.
This is illustrated by the results of the most serious damage of F1 generation of male rats’reproductive function, and with the increase of genetic algebra, damage degree showed a trend of decline. A:double head;B:head;C:curve;D:fold.
Table 8.
Three kinds of DNA methyltransferase mRNA expression.
Fig 5.
Control group and DEHP experimental group offspring (F1-F4) male rat’s DNMT1 expression.
High expression of dnmt1 in the F1, F2 generation significantly reduced, F3 and F4 generation basic without expression. A:Control;B:F1;C:F2;D:F3 E:F4 (×200)
Fig 6.
Control group and DEHP experimental group offspring (F1-F4) male rat’s DNMT3a expression.
High expression of dnmt1 in the F1 and F2 generation, F3 significantly reduced and F4 generation basic without expression. A:Control;B:F1;C:F2;D:F3 E:F4 (×200)
Fig 7.
Control group and DEHP experimental group offspring (F1-F4) male rat’s DNMT3a expression.
Result shows that with the increase of genetic algebra, the expression level of DNA methyltransferase gradually lowered, the testicular damage degree is reduced.
Fig 8.
Control group and DEHP experimental group cryptochidism group compared to control group of biological process up regulation of GO Biological Process Classification.
Fig 9.
Control group and DEHP experimental group cryptochidism group compared to control group of biological process down regulation of GO Biological Process Classification.
Fig 10.
Control group and DEHP experimental group cryptochidism group compared to control group of biological process down regulation of pathway analysis.