Table 1.
Average dTm values for mutations tested by both DLP and SMB rpoB assays.
Table 2.
Average dTm values for mutations tested by both DLP and SMB gyrA, eis promoter and rrs assays.
Fig 1.
Analytical sensitivity of the SMB assays.
Melting temperature first derivative peak profiles of the targets tested for the SMB probes on serial dilutions of M. tuberculosis H37Rv genomic DNA. Input DNA amount is indicated as genome equivalents.
Fig 2.
Analytical sensitivity of the DLP assays.
Melting temperature first derivative peak profiles for all the targets for the DLP probes on serial dilutions of M. tuberculosis H37Rv genomic DNA. Input DNA amount is indicated as genome equivalents.
Fig 3.
Limit of detection (LOD) of the individual SMB and DLP assays on M. tuberculosis H37Rv genomic DNA.
Assays were performed with a dilution series of genomic equivalents as indicated. Assay success rates for individual probes and the entire assay over the input DNA dynamic range are shown. Panel (A) SMB probe and assay performance; Panel (B) DLP probe and assay performance.
Fig 4.
Limit of detection (LOD) of the individual SMB and DLP assays on sputum spiked with M. bovis BCG CFU.
Both SMB and DLP assays were performed on DNA isolated from sputum spiked with a serial dilution of M. bovis BCG CFU as indicated. Assay success rates for individual probes and the entire assay over the dynamic range of spiked CFU/ml are shown. Panel (A) SMB probe and assay performance; Panel (B) DLP probe and assay performance.
Fig 5.
Analytical sensitivity of SMB and DLP probes on mutant DNA from clinical samples.
Success rates for the detection of selected mutants by the SMB and the DLP assays are shown. Panel (A) rpoB mutants; panel (B) gyrA mutants and panel (C) rrs/eis mutants. Solid lines show SMB assay performance and dotted lines show DLP assay performance.
Table 3.
Comparative detection success at different concentrations of mixed wild-type and representative mutant DNA.