Fig 1.
Types of Bombus brasiliensis Lepeletier, 1836 and Bombus bahiensis sp. n.
Lectotype of B. brasiliensis—A: lateral view of head, mesosoma and metasoma; B: dorsal view of mesosoma and metasoma. Holotype of B. bahiensis sp. n.—C: lateral view of head, mesosoma and metasoma; D: dorsal view of mesosoma and metasoma.
Table 1.
Specimens belonging to GenBank and BOLD Systems databases, which were used in analyzes with the COI gene.
Fig 2.
Network of haplotypes for Bombus brasiliensis Lepeletier, 1836 and Bombus bahiensis sp. n.
Median-joining network of haplotypes for B. brasiliensis and B.bahiensis sp. n. The B. brasiliensis on isolated moutaintops in southeastern Brazil are coloured of different shades of green and the B. brasiliensis continuously distributed are coloured of different shades of blue. B.bahiensis sp. n. is coloured yellow. All lines joining haplotypes are one mutation step long, except for three of them, marked with “a”, “b” and “c”, which are three, two and 38 steps long, respectively.
Fig 3.
Distribution of Bombus brasiliensis Lepeletier, 1836 and Bombus bahiensis sp. n.
Records for B. bahiensis are represented by triangles (the black triangle, the gray triangle and the gray triangle with black point represents a population used in mtDNA analyzed, a population used in morphologic analyzed and a population inferred to belong to the species based on descriptions in Moure &Sakagami [23], respectively); records for B. brasiliensis are represented by circles (the black circle denote sites represented by mtDNA). Records were compiled from the literature [23, 60, 73] and from specimens deposited at UFMG and/or listed in S1 Table. Acronyms represent Brazilian states, as follows: BA = Bahia; ES = Espírito Santo; MG = Minas Gerais; RJ = Rio de Janeiro; SP = São Paulo; PR = Paraná; SC = Santa Catarina; RS = Rio Grande do Sul; MT = Mato Grosso; GO = Goiás; MS = Mato Grosso do Sul; DF = Distrito Federal. Localities mentioned in the text are indicated. The stippled line indicated by black arrow represents the Doce river.
Table 2.
Genetic distances for COI sequences (%) within species of bumblebees.
Fig 4.
Phylogenetic relationships among Brazilian bumblebees obtained from concatenated phylogenetic analyses performed with CytB and COI sequences, using Bayesian inference and all specimens available for each species.
Values shown under each branch are posterior probabilities. The values shown above each branch are bootstrap values represented here by Maximum Parsimony (MP)/ Maximum Likelihood (ML), because an identical topology was obtained with MP and ML. An analysis employing only unique haplotypes yields the same topology. “*” Represents the same values for MP and ML. Bombus morio (Swederus, 1787) was used as outgroup. The collapsed branch includes all Bombus brasiliensis haplotypes found on the haplotype network from Fig 2 and S1 Fig.