Table 1.
Mating trials performed for fitness and reproductive compatibility assessment.
Table 2.
Adult body size (mm) of individuals from Leptodiaptomus sicilis and L. cf. sicilis.
Fig 1.
Simplified neighbor-joining tree of COI sequences.
The branch of Leptodiaptomus cf. sicilis comprises individuals from Atexcac, La Preciosa, El Carmen and Quechulac lakes (Oriental Basin) and is compared to congeneric species using K2P genetic distances. Mastigodiaptomus albuquerquensis was used as outgroup species. Numbers over branches indicate percent bootstrap support (1000 replicates).
Fig 2.
Nested clade design for the haplotype network of COI sequences of Leptodiaptomus cf. sicilis populations.
A-O: Haplotypes found. Circle size represents haplotype frequency; color indicates the lake where haploptypes are distributed. Black: El Carmen; dark gray: Atexcac; light gray: La Preciosa; white: Quechulac. Haplotype (E) corresponding to the most extended and probably ancient one. White, unlabeled circles represent inferred haplotypes. Informative clades are indicated with numbers (x.y).
Table 3.
Genetic divergence (mean ± SE) between distinct clades of L. cf. sicilis populations and other Leptodiaptomus species.
Fig 3.
Reaction norms for the proportion of hatching in resting eggs from three L. cf. sicilis populations at several salinities.
Low: 1.1 g L-1; Medium: 6.5 g L-1 for La Preciosa and Atexcac, 3.8 g L-1 for El Carmen; High: 9.0 g L-1.
Fig 4.
Survivorship curves of three populations of L. cf. sicilis at home and alternative salinities.
Values are means ± SE; letters indicate significant differences among populations according to the log-rank tests (P ≤ 0.05).
Fig 5.
Development (time to molting from CIII to adult stage) of the three studied populations of L. cf. sicilis at home and alternate salinities.
Values are means ± SE.
Fig 6.
Intra-population mating at home and alternative salinities.
(A) Percentage of copulated females (spermatophore attached to the genital pore). (B) Egg ratio (number of eggs/number of females); (C) Relative hatching (number of nauplii/number of females); (D) Hatching success (nauplii/eggs). Values are mean ± SE.
Fig 7.
Performance of populations of L. cf. sicilis at alternate salinities relative to home conditions.
Fitness at home conditions = 1, pointed line.
Fig 8.
Performance of immigrants from populations of L. cf. sicilis in different salinities relative to resident populations. Resident population between brackets. Fitness of resident populations = 1, pointed line.
Fig 9.
Reproductive compatibility among populations in a common garden experiment (3.8 g L-1).
(A) Percentage of copulated females (spermatophore attached to the genital pore). (B) Egg ratio (number of eggs/number of females); (C) Relative hatching (number of nauplii/number of females); (D) Hatching success (nauplii/eggs). Values are mean ± SE.