Table 1.
Effect of copper stress on yield of copper-binding proteins in the rice roots of varieties B1139 and B1195.
Fig 1.
Representative 2-DE maps of the rice B1139 and B1195 roots copper-binding proteins eluted from a Cu-IMAC column with elution buffer containing 40 mmol imidazole.
The 7 days seedlings were treated with 8 μmol Cu2+ for 3 days. Seedlings without any treatment, grown in a full-strength nutrient solution were used as a control (0.32 μmol Cu2+). 100 μg of copper-binding protein were loaded onto IPG dry strips (17 cm, pH 4–7 linear gradient; Bio-Rad) during the rehydration step (13 h), followed by focusing for a total of 6,000 V·h using a PROTEAN IEF CELL (Bio-Rad). SDS-PAGE in the second dimension was carried out using 12% SDS—polyacrylamide gels. The protein spots were visualized by mass spectrometry-compatible silver staining.
Fig 2.
Enlargements of the framed areas shown in Fig 1.
The framed regions A-E in Fig 1 are enlarged and compared in detail. The arrows indicate the distinct copper-binding proteins between rice B1139 and B1195 roots.
Table 2.
Differentially expressed copper-binding proteins identified by MS/MS.
Table 3.
Potential Cu-binding motifs of identified proteins.