Fig 1.
Morphological and physiological changes in seedlings of two wheat (Triticum aestivum L.) cultivars, ‘Chinese Spring’ (CS) and ‘Ningchun 47’ (NC47), during 48 h of PEG-mediated drought stress.
(A) Morphological changes of wheat seedlings in response to different PEG6000 concentration treatments. (B) Leaf relative water content (RWC) analysis. (C) Proline content analysis. (D) Soluble sugar content analysis.
Fig 2.
Proteome maps of two spring wheat varieties.
(A) CS under the control condition. (B) NC47 under the control condition. The numbered protein spots were identified by MALDI-TOF/TOF, and yellow numbers represent the common DAP spots in NC47 and CS.
Fig 3.
Functional distribution of identified differentially accumulated proteins in CS and NC47.
Fig 4.
Principal components analysis (PCA) of the set of 101 DAP spots in NC47 and CS.
(A) PCA of individual experimental samples in NC47. (B) PCA of 69 differentially accumulated protein spots in NC47. (C) PCA of individual experimental samples in CS. (D) PCA of 77 differentially accumulated protein spots in CS. Red spots indicate an upregulated protein; green spots indicate a downregulated protein. CS-0, CS-15, CS-20, CS-25 and CS-30 represent the 0, 15%, 20%, 25% and 30%, respectively, of the PEG6000 concentration treatment in CS. NC47-0, NC47-15, NC47-20, NC47-25 and NC47-30 represent the 0, 15%, 20%, 25% and 30%, respectively, of the PEG6000 concentration treatment in NC47.
Fig 5.
Hierarchical cluster analysis of DAP spots in NC47 and CS.
Red colour indicates a positive abundance in protein spots; green colour denotes a negative abundance in protein spots. (A) Cluster analysis of 69 differentially accumulated proteins in NC47. (B) Cluster analysis of 77 differentially accumulated proteins in CS. 0, 15%, 20%, 25% and 30% represent the PEG6000 concentration treatment.
Table 1.
Comparison of some important DAPs in seedling leaves of NC47 and CS.
Fig 6.
Network of key DAPs in NC47 involved in drought adaptation and tolerance.
Interactions of the DAPs are extracted by searching the STRING database with a confidence cutoff of 0.700. The interaction network is reconstructed using the Cytoscape software. Red colour represents the upregulated proteins and green colour shows the downregulated proteins in NC47 under high PEG6000 concentration stress. Blue colour indicates the signal transduction-related protein.
Fig 7.
Drought stress-mediated changes in metabolic pathways of NC47 seedling leaves.