Fig 1.
Homografting in A. thaliana plants.
(A) Representative Arabidopsis plants selected for floral stem wedge-grafting (scale 2.5 cm). (B) Grafted plants (scale 2.5 cm) selected for harvesting the newly emerged un-opened flower buds and leaves (scale 500 μm) from the side branches of scion (up) and rootstock (down). The arrow shows rootstock in the plants. (C) A floral stem graft (10 DAG) showing wedge junction (scale 5 mm). (D) A longitudinal section through the floral stem graft (10 DAG) showing callus proliferation (arrow) near the wedge junction (scale 1 mm). (E) A transverse section from middle of the floral stem graft (10 DAG) (scale 500 μm). (F) Siliques (scale 1 mm) and seeds (scale 500 μm) of rootstock, and (G) scion. (H) Bar diagram representing length, and (I) seed number in mature siliques of rootstock and scion. The error bars indicate standard error in three biological replicates.
Fig 2.
Venn diagram showing differentially expressed genes in flower bud and leaf (scion vs. rootstock; ≥ 2 fold change; p ≤ 0.05) (U = up-regulated, D = down-regulated).
The details of the genes have been given in S1 Table.
Table 1.
MapMan functional categories (BINs) for significantly up-regulated (2 ≥ fold) genes in the flower buds of scion and rootstock.
Table 2.
MapMan functional categories (BINs) for significantly up-regulated (2 ≥ fold) genes in the leaves of scion and rootstock.
Table 3.
AgriGO categories for significantly up-regulated (2 ≥ fold) genes in the flower buds of scion and rootstock.
Table 4.
AgriGO categories for significantly up-regulated (2 ≥ fold) genes in the leaves of scion and rootstock.
Table 5.
KEGG categories for significantly up-regulated (2 ≥ fold) genes in the flower buds of scion and rootstock.
Table 6.
KEGG categories for significantly up-regulated (2 ≥ fold) genes in the scion leaf.
Fig 3.
A heatmap of differentially expressed transcripts related to transcription factors in flower bud and leaf (scion vs. rootstock; ≥ 2 fold change; p ≤ 0.05).
The color scale at the top of each heat map shows expression values in fold change. The details of the genes have been mentioned in S2 Table.
Fig 4.
MapMan visualization of differentially expressed genes (scion vs. rootstock; p ≤ 0.05) assigned to the functional category of hormone metabolism in (A) flower bud, and (B) leaf.
The details of the genes have been mentioned in S3 table.
Table 7.
Genes (other than TFs) showing at least 8-fold (bold) differential expression (scion vs rootstock) in flower buds and/or leaves.