Table 1.
Summary of differentially expressed genes in total CD8 T-lymphocytes in HH patients with a low CD8 phenotype (n = 6) relative to HH patients with a normal/ high CD8 phenotype (n = 4).
Fig 1.
Gene expression analysis in CD8+ T-cell subpopulations.
A) Gating strategy used to discriminate the CD8+ T subpopulations of naïve, central memory and effector memory cells. B) Relative mRNA expression levels of CCR7, LEF1, ACTN1, FOSL2, P2RY8 and NAA50 in isolated CD8+ T subpopulations. Statistical significant differences were calculated by T-test between HH patients and controls in each subpopulation.
Table 2.
Comparisons of CD4+ and CD8+ T-lymphocyte percentages in different cell cycle phases between HH patients and controls.
Fig 2.
Genome-wide expression analysis of CD8+ T lymphocytes from Hfe-/- and wild type mice.
A) Venn diagram with comparative analysis between the selected genes found to be differently regulated by the two genotypes under the same iron diet condition. B) Differential gene expression of Hfe-/- mice with normal and high iron diet relatively to C57BL/6 mice under the same iron diet conditions. Fold Change of the genes found to be differently expressed (T-test, p<0.05) and with fold change >1.8 are represented.
Fig 3.
Hepatic iron concentration of Hfe-/- and C57BL/6 mice under a normal or a high-iron diet.
Values are expressed as mean ± standard deviation. Statistical significant differences were calculated between mice genotype groups under the same diet condition (T-test * P<0.05 and ***P<0.001).
Fig 4.
S100a8 and S100a9 expression in CD8+ T cells from HH patients and controls.
A) S100a8 and S100a9 mRNA expression in isolated CD8+ T lymphocytes from HH patients and normal controls. Statistical significance was obtained with paired analysis of patient-control of the day using the non-parametric Wilcoxon test, *p<0.05. B) Median fluorescence intensity of S100a9 expression in CD8+ T cells from peripheral blood from HH patients and normal controls. Statistical significance was obtained with paired analysis of patient versus control of the day using the parametric T-test, *p<0.0001.