Fig 1.
Growth analysis of the DvH strains.
Growth curves of the wild-type and the three deletion mutant strains under anaerobic conditions (black symbols) or with constant 0.02% O2 gas mixture sparging (open symbols). Data arose from five independent growth experiments.
Table 1.
Growth parameters for the Desulfovibrio vulgaris Hildenborough strains.
Fig 2.
Variability in the cell length of the various DvH strains.
Comparison of the distribution of the cell length in cultures under anaerobic conditions (black bars) and with constant 0.02% O2 gas mixture sparging (striped bars). The average cell length was determined from two independent cultures (>200 cells were counted in each experiment). Statistical analysis using One-way ANOVA were performed to reveal significant differences between distributions (p<0.05), which were mentioned by an asterisk.
Fig 3.
Lactate consumption and acetate production in the DvH strains.
Quantification of consumed lactate (A) and produced acetate (B) after 40h growth under anaerobic conditions (black bars) or with constant 0.02% O2 gas mixture sparging (striped bars) in medium C (with 37mM of lactate as initial concentration).
Fig 4.
Biomass formation under various culturing conditions.
Final biomass for the four DvH strains cultured under anaerobic conditions only (black bars), with constant 0.02% O2 gas mixture sparging (striped bars) and after growth resumption (open bars). Data are mean values of five independent experiments + SD. Statistical analysis using t-test was used to compare means. Significant differences (p< 0.05) are mentioned by an asterisk.
Fig 5.
Molar growth yield for the four strains under various growth conditions.
Molar growth yield on lactate for the wild-type and deletion mutant strains cultured under anaerobic conditions (black bars) and with constant 0.02% O2 gas mixture sparging (striped bars). Molar growth yields are expressed in 1012 cells/mole of substrate.
Table 2.
Quantification of cells able to divide and average division parameters of the wild-type and deletion mutant strains after oxygen exposure.
Fig 6.
Variability of the second doubling time for the four strains after oxygen exposure.
Box and whiskers plot representing the variability in the distribution of the second doubling time of the various DvH strains cultured with constant 0.02% O2 gas mixture sparging followed by a switch to anaerobic condition. These distribution patterns arose from at least 2 independent cultures (> 200 cells each).
Fig 7.
Protected PFOR rate in the four strains.
Quantification of the rate of protected PFOR during the exponential growth phase under anaerobic (black bars) and with constant 0.02% O2 gas mixture sparging (striped bars). Statistical analysis using Mann and Whitney test was used to compare means. Significant differences (p< 0.05) are indicated by an asterisk. All values are related to the PFOR protected rate in the wild-type strain under anaerobic conditions (value arbitrary set to 1).