Table 1.
Subject Demographics.
Fig 1.
Overview of the stable-isotope labeling strategy employed to measure hepatic and plasma protein turnover in human subjects with chronic liver disease.
Table 2.
List of 20 hepatic proteins with relative abundances observed to most significantly positively correlate with histological fibrosis score.
Table 3.
List of 10 hepatic proteins with fractional synthesis rates (FSRs) that most significantly positively correlate with histological fibrosis score.
Fig 2.
Hepatic Protein Turnover Rate vs Abundance.
Heat maps displaying hepatic protein FSR (A) and relative abundance (B) for 6 human subjects with chronic liver disease. Protein data are row-scaled, with the columns representing each subject sorted by increasing histological fibrosis score. Abbreviation: fractional synthesis rate (FSR).
Fig 3.
Comparison of Hepatic Collagen Kinetics and Histologic Fibrosis Score.
(A) Linear regression of hepatic type I collagen FSR (% new per day) and histopathologic fibrosis score in human subjects with chonic liver disease. (B,C) Bar graphs depicting the relative abundance of unlabeled (old) and labeled (new) hepatic type I collagen (B) and type III collagen (C) per unit mass of liver protein, normalized to 30 days of labeling in vivo. Collagen FSR values (% new per 30 days) are displayed above each bar. Values shown represent the subject or the mean of subjects with each histopathology score. Abbreviation: fractional synthesis rate (FSR).
Fig 4.
Comparison of Plasma Lumican FSR to Hepatic Collagen Kinetics and Histopathologic Fibrosis Score.
(A) Linear regression of FSR of plasma lumican vs FSR of hepatic collagen in human subjects with chronic liver disease. (B) Linear regression of plasma lumican FSR vs histopathologic fibrosis score in human subjects with chronic liver disease. (C) Comparison of FSRs of liver and plasma lumican in individual subjects in whom liver values were measurable. Abbreviation: fractional synthesis rate (FSR).
Fig 5.
Comparison of Plasma TGFBI FSR to Hepatic Collagen Kinetics.
(A) Linear regression of FSR of plasma TGFBI vs FSR of hepatic type I collagen in human subjects with chronic liver disease. (B) Linear regression of FSR of plasma TGFBI vs FSR of hepatic type III collagen in human subjects with chronic liver disease. (C) Comparison of FSRs of liver and plasma TGFBI in individual subjects in whom liver values were measurable. Abbreviations: fractional synthesis rate (FSR); transforming growth factor beta-induced protein (TGFBI).