Fig 1.
(A) GroPIns. (B) inositol-d6 (IS).
Fig 2.
Representative LC-MRM chromatograms.
(A) LOQ (3 ng/ml) of GroPIns in water. (B) GroPIns at the highest standard concentration (3,000 ng/ml) in water. (C) Blank sample after injection of the highest concentration of GroPIns (3,000 ng/ml).
Fig 3.
Representative LC-MRM chromatogram of GroPIns and of the IS.
(A) Cell extract of Raw264.7. (B) Cell extract of Raw 264.7 spiked with standard GroPIns.
Fig 4.
Linearity of GroPIns and analyte concentration in Raw 264.7 cell extract.
(A) Standard addition calibration in matrix. (B) Internal standard calibration in water. Error bars are expressed as SD.
Table 1.
Matrix effect in Raw 264.7, RBL-2H3 and Jurkat T-cell extracts.
Table 2.
Intra-day and inter-day precision and accuracy in Raw 264.7 cell extract.
Table 3.
Linearity and GroPIns concentration in RBL-2H3 and Jurkat T-cell extracts by internal standard calibration and standard addition method.
Table 4.
Intra-day precision and accuracy in RBL-2H3 and Jurkat T-cell extracts.
Table 5.
Inter-day precision and accuracy in RBL-2H3 and Jurkat T-cell extracts.
Table 6.
GroPIns levels in untreated and stimulated cells measured by external calibration method.