Table 1.
Bacterial strains used in this study.
Fig 1.
Transmission electron microscopy image of gold nanoparticles.
Particles had varying sizes and shapes with an average diameter of 20±5.26 nm.
Fig 2.
UV/Vis spectra of colloidal gold and the antibody-gold conjugate.
Blue: colloidal gold solution; green: the antibody-colloidal gold conjugate.
Fig 3.
Colloidal gold immunochromatographic strips showed positive results for different serotypes of R. anatipestifer strains.
1–12: R. anatipestifer serotype 1 strains CH3, CH1, CQ1, CQ3, CQ4, CQ5, JY4, YXb12, NN2, NJ1, NJ4 and YL4 respectively; 13–23: R. anatipestifer serotype 2 strains JY1, SC2, NJ3, Yb2, Th4, YXb1, NN1, NN5, GD3, GD4 and GD5 respectively; 24: R. anatipestifer serotype 6 strain P2123; 25: R. anatipestifer serotype 8 strain D26220; 26–28: R. anatipestifer serotype 10 strains YXb11, HXb2 and YXL1; 29: R. anatipestifer serotype 12 strain 8785; 30–31: R. anatipestifer serotype 15 strains D743 and NN6; 32–33: Serotype not determined R. anatipestifer strains JY6 and GD6. 34: R. anatipestifer serotype 1 strain WJ4 (CGMCC 5264).
Fig 4.
Colloidal gold immunochromatographic strips showed negative results for Escherichia coli, Salmonella enterica and Pasteurella multocida.
1: E. coli reference strain CVCC 1547; 2–3: E. coli serotype O1 strains APEC O1 and DE171; 4–5: E. coli serotype O2 strains DE14 and DE17; 6: E. coli serotype O18 strain CE66; 7–8: E. coli serotype O78 strains DE48 and DE56; 9–13: S. enterica strains CVCC1805, CAU0118, JXb1, CVCC 3384, and CVCC 519 respectively; 14: P. multocida strain CVCC 493.
Fig 5.
Sensitivity of colloidal gold immunochromatographic strips for detecting R. anatipestifer strain WJ4.
1–6, WJ4 at 108, 107, 106, 105, 104, 103 CFU/strip respectively.
Table 2.
Specificity and sensitivity of colloidal gold immunochromatographic strip after 4°C storage.
Table 3.
Comparison of colloidal gold immunochromatographic strips, m-PCR and agglutination test for detecting Riemerella anatipestifer.