Table 1.
Demographical characteristics.
Table 2.
Synovial fluid vs. blood levels.
Table 3.
Correlations between parameters detected in serum and synovial fluid.
Fig 1.
ApoA1-induced IL-6 expression after 24h using primary human chondrocytes.
A) IL-6 expression after stimulation by ApoA1 (30μg/mL) in the presence or not of polymyxin B (PB) (1μg/mL). B) Dose response with ApoA1 (human source; 50-15-3μg/mL) and rhSAA (5–2.5μg/mL). C) ApoA1 (human source; 56–5.6μg/mL) with or without HDL (260-50-3μg/mL). D) rhSAA (3.5μg/mL) with or without HDL (260-50-3μg/mL). E) ApoA1 (human source; 56–5.6μg/mL) with or without LDL (180-30-2μg/mL). D) rhSAA (3.5μg/mL) with or without LDL (180-30-2μg/mL). *b,*d,*f,*h and *j statistically different to “a”, “c”, “e”, “g” and “i”, respectively. Data were expressed as means ± SEM (n = 3). * P< 0.05 by Mann–Whitney test.
Fig 2.
Endotoxin contamination and polymyxin B efficacy using primary human chondrocytes and fibroblast-like synoviocytes (FLS).
A) Primary human chondrocytes and B) FLS were stimulated with rhApoA1 (30μg/mL) or rhSAA (3.5μg/mL) or LPS (10-1-0.1 μg/mL) in the presence or not of polymyxin B (1μg/mL).
Fig 3.
ApoA1-induced IL6, MMP-1 and MMP-3 expressions in the presence of lipoproteins after 24h using primary human chondrocytes and fibroblast-like synoviocytes (FLS).
A) Primary human chondrocytes and B) FLS were stimulated with ApoA1 (human source) (56μg/mL), rhSAA (3.5μg/mL), HDL (260μg/mL) and LDL (180μg/mL). Data were expressed as means ± SEM (n = 3). * P< 0.05 by Mann–Whitney test.
Fig 4.
TAK242 reduced ApoA1-induced IL-6, MMP-1 and MMP-3 expressions in human primary chondrocytes and fibroblast-like synoviocytes (FLS).
A) Primary human Chondrocytes and B) FLS were stimulated with ApoA1 (30μg/mL) in the presence or not of several inhibitors: lipoxin A4 (LX4; 1μM), anti-SR-B1 antibody (5μg/mL) and its isotype IgG1 control antibody (5μg/mL), anti-RAGE (10μg/mL) and its isotype IgG control antibody (10μg/mL) and TAK242 (1μM) for FPRL1, SR-B1, RAGE and TLR4 receptors, respectively. Polymyxin B was used at a concentration of 1μg/mL. *b and *h statistically different to “a” and “g”, respectively. Data were expressed as the means ± SEM (n = 3). * P< 0.05 by Mann–Whitney test.