Fig 1.
Schematic representation of annotated genes of the OXPHOS pathway in gilthead sea bream.
Mitochondrial-encoded genes are highlighted in bold. Assembly factors are indicated in italics.
Table 1.
A. PCR-array layout (88 genes) with extra wells for housekeeping genes (ACTB) and general controls of PCR performance.
Table 2.
Growth and biometric parameters of fed (CTRL group) and fasted gilthead sea bream.
Fig 2.
Fold-change of differentially expressed genes (P< 0.05) in the liver tissue of fasted fish.
Fish were fed with a commercial diet to visual satiety (Control, CTRL group) or remained unfed for ten days (fasted group). Data of fold-change are relative to the CTRL group. The intensity of green boxes represents the degree of down-regulation. Mitochondrial-encoded catalytic subunits are in bold and red. Nuclear-encoded catalytic subunits are in red. Nuclear-encoded regulatory subunits are in black. Nuclear-encoded assembly factors are in blue and italics.
Fig 3.
Fold-change of differentially expressed genes (P< 0.05) in the white skeletal muscle of fasted fish.
Fish were fed with a commercial diet to visual satiety (Control, CTRL group) or remained unfed for ten days (fasted group). Data of fold-change are relative to the CTRL group. The intensity of red boxes represents the degree of up-regulation. Nuclear-encoded catalytic subunits are in red. Nuclear-encoded regulatory subunits are in black. Nuclear-encoded assembly factors are in blue and italics.
Fig 4.
Fold-change of differentially expressed genes (P< 0.05) in the cardiac muscle of fasted fish.
Fish were fed with a commercial diet to visual satiety (Control, CTRL group) or remained unfed for ten days (fasted group). Data of fold-change are relative to the CTRL group. The intensity of red boxes represents the degree of up-regulation. Mitochondrial-encoded catalytic subunits are in bold and red. Nuclear-encoded catalytic subunits are in red. Nuclear-encoded regulatory subunits are in black. Nuclear-encoded assembly factors are in blue and italics.
Fig 5.
Western blot of COX4 in liver, white skeletal muscle and cardiac muscle of CTRL and fasted fish.
Representative Western blots of tissue protein samples (20 μg) of CTRL and fasted individuals, and integrated intensities of bands. For each tissue, data are expressed as the percentage of intensity in comparison with the CTRL group samples (100% value). Data are represented as mean ± SEM (n = 6) and statistically significant differences between CTRL and fasted groups are indicated (*, P<0.05; Student t-test).