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Fig 1.

Sampling context.

(a) A map of the Yilgarn Craton in Western Australia showing the location of the acid salt lake sampled. (b) At the time of sampling, the acid salt lake was at a stage of evapoconcentration with a maximum depth of 20 cm. (c) Bedding plane of the sampling site, below a 5mm efflorescent halite crust. (d) Within the bulk sediment sample, three distinctive layers were subsampled for VNIR spectroscopy and SEM analysis (orange, green, and black). Arrow indicates stratigraphic up.

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Fig 1 Expand

Fig 2.

XRD results.

(a) XRD pattern from the bulk sediment sample with derived bulk mineralogy is shown in the pie chart. (b) XRD pattern after sieving to the <4 μm fraction showed no change upon ethylene glycol treatment, pointing to an absence of smectite clay minerals. (c) VNIR spectra showed aluminum phyllosilicate in all three discrete layers in the sample with variations in the type and quantity of iron oxides. Small sharp absorptions from 0.6–0.7 μm may be due to pigments in microbes.

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Fig 2 Expand

Fig 3.

SEM results.

(a) Gypsum and chloride precipitates on a silicate substrate (bulk sample). (b) Iron oxide precipitates (orange sample). (c) Magnesium sulfates on a silicate substrate surrounded by precipitated chloride (black sample).

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Fig 3 Expand

Table 1.

Read statistics.

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Table 1 Expand

Fig 4.

Functional assignment of reads.

Groupings are shown for the predominant NOG, KO, and COG identifiers.

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Fig 5.

Taxonomic diversity of the sediment.

Gray shading indicates the proportion of sequences that could not be assigned to a known phylum, class, or order.

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Table 2.

Sequences associated with sulfur metabolism.

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Table 2 Expand