Table 1.
List of primers used in the quantitative and semi-quantitative RT-PCR.
Fig 1.
Effect of stress treatment on (A) GSH content and (B) GSH:GSSG ratio in Col-0 and pad2.1.
Data are presented as mean ± SE (n = 3). Lower case letters indicate significant difference from Col-0 at aP<0.05, bP<0.01 and cP<0.001 (Student-Newman-Keuls multiple comparison test).
Fig 2.
Heat map with hierarchical cluster tree for differentially expressed genes.
Tree classified on the basis of gene expression. Over-expressed (log2 fold change > = 1) and under-expressed (log2 fold change < = -1) genes are shown by red and green colour respectively.
Fig 3.
DAVID functional annotation categories of differentially expressed genes.
Significantly enriched categories for (A) up-regulated genes and (B) down-regulated genes in pad2.1.
Fig 4.
Hierarchical tree graph of over-represented GO terms in down-regulated genes by singular enrichment analysis generated by agriGO.
Boxes in the graph show GO terms labelled by their GO ID, term definition and statistical information. The significant terms (adjusted P<0.05) are marked with color, while non-significant terms are shown as white boxes. The degree of color saturation of a box correlates positively with the enrichment level of the term. Solid, dashed and dotted lines represent two, one and zero enriched terms at both ends connected by the line, respectively. The rank direction of the graph runs from top to bottom.
Fig 5.
In response to combined stress treatment, (A) Functional annotation of the differentially expressed gene in Col-0 and pad2.1 through MapMan.
Differentially expressed genes related to (B) hormones, (C) metabolic pathways and (D) secondary metabolites in Col-0 and pad2.1.
Fig 6.
MapMan analysis of different (A) transcription factors (B) cellular redox maintaining genes.
Up-regulated genes in blue and down-regulated genes in red colour respectively.
Fig 7.
Identified genes related to (A) phenylpropanoid and lignin (B) ET biosynthetic pathways by MapMan analysis.
Up-regulated genes in red and down-regulated genes in blue colour respectively.
Fig 8.
Functionally categorized (A) down-accumulated and (B) up-accumulated proteins in response to combined stress treatment in pad2.1.
Fig 9.
Validation of some differentially expressed genes of microarray experiments by (A) comparative proteomic analysis (B) Quantitative RT-PCR.
Data are presented as mean ± SE (n = 3). P<0.05 (Student’s t-test).
Table 2.
MALDI TOF-TOF MSMS based identification of proteins which validated differential expression of five genes in pad2.1 in response to combined stress.
Fig 10.
Stress response overview of transcriptome altered in response to combined stress treatment in pad2.1 as visualized by MapMan analysis.