Fig 1.
Lighting and motor activity patterns.
Top: Double plotted graphs corresponding to light patterns, where the dark areas correspond to darkness. Bottom: Motor activity of a representative animal of each group.
Fig 2.
Weight, motor activity and fasting blood glucose, insulin and leptin levels.
Adv + Del: Advanced and Delayed group. (*p<0.05, n = 8).
Fig 3.
Clock genes in hypothalamus, liver, WAT and BAT.
mRNA expression of genes involved in the core clock system. (*p<0.05, n = 7–8).
Fig 4.
Gene expression analysis in liver and hypothalamus.
(A) Expression of genes involved in lipid and glucose metabolism in the liver. (B) Lipid metabolism, inflammation, neuropeptides and Endoplasmic Reticulum (ER) stress genes were analysed in the hypothalamus. (*p<0.05, n = 7–8).
Fig 5.
Advanced light shifts lead to histological and gene expression changes in BAT and WAT.
BAT (A) mass, (B) mRNA expression and (C) histology (H&E staining) of advanced and control rats. (D) WAT mRNA gene expression. (* = p<0.05, n = 7–8).