Fig 1.
Reactions of apple leaves toward the fungal pathogen A. alternata.
A: Observation of the reactions of resistant (R) and susceptible (S) leaves 0 h and 48 h after inoculation. CK: The control samples were treated with water instead of inoculums. B: Relative changes in symptoms of infected host cells before and after treatment with pathogen visualised by transmission electron micrographs (Bars = 5 μm) Chl: Chloroplast; V: Vacuole; Cw: Cell wall; N: Nucleus; S: Starch grains.
Fig 2.
2-DE analysis of proteins induced by the A. alternata pathogen in resistant (R) and susceptible (S) leaves.
Total protein (800 μg) was separated on 2D gels (pH 4–7) and stained with CBB R-350. Approximate molecular masses and pIs are indicated in the margins. Circles indicate the 43 proteins identified by MALDI-TOF-TOF/MS that changed in abundance more than 1.5-fold between controls and treated samples. The affected proteins are numbered, and the numbers correspond to the numbers in Fig 3. This figure represents three biological replicates.
Fig 3.
Differentially expressed proteins identified from resistant (R) and susceptible (S) leaves.
Fig 4.
Venn diagram showing the distribution of protein spots expressed as indicated in Fig 2.
Fig 5.
Functional categorisation of the identified proteins that are differentially regulated in resistant (R) and susceptible (S) leaves infected with the A. alternata pathogen.
A total of 43 identified proteins were assigned to the functional categories. The Roman numerals of the categories correspond to the functional categories described in Fig 3. The percentage represents the proportion of proteins in each category.