Table 1.
Ploidy levels for five tissues from honey bee (Apis mellifera) workers at different days post emergence (0 to 55 days of age).
Fig 1.
Least square estimates of mean ploidy levels post-emergence (intercept), and change of ploidy over time (slope) for five different worker honey bee (Apis mellifera) tissues: Brain, thoracic (flight) muscle, leg muscle, Malpighian tubules, and stinger.
Adult honey bee workers were collected at different days post emergence (See Methods for details).
Table 2.
Least squares estimates of endopolyploid levels in newly emerged honey bee workers (i.e., "Intercept") and rate of change of endopolyploidy per day (i.e., "Slope") for the five tissues studied.
Fig 2.
Histograms showing fluorescence peaks centered at mean fluorescence channels 25, 50, 100, 200, and 400 produced by 2N, 4N, 8N, 16N and 32N nuclei isolated from flight muscle of (A) a 3-day-old and (B) a 27-day-old worker bee.
The count of nuclei under each peak and the average ploidy level are given immediately below each histogram. The reported significant reduction of ploidy with age (P < 0.01) is reflected in the reduced 8N peak and 16N peak in the older bee.