Fig 1.
Lifecycle and anatomy of Lucilia sericata.
A. Lifecycle of the blow-fly from eggs through to adult. B. Anatomy of a maggot showing the location of the gut, crop and salivary glands. The gut was sampled from the whole specimen shown. The crop is the round organ filled with air-bubbles which was further dissected away from the mouthparts, highlighted by the dotted-line circle. The salivary glands are the Y-shaped tubes shown here encircled by dotted lines, still attached to the mouthparts.
Table 1.
Summary of counts per library and unique reads once self-aligned.
Table 2.
Summary of matched reads to different types of non-coding RNA.
Fig 2.
Summary of insect tRNA counts in L. sericata tissue libraries.
The proportion of each tRNA in the different tissue libraries as normalised to the total number of insect tRNA matches in that sample. tRNAs are denoted by their amino acid name.
Fig 3.
Abundance of miRNA counts in L. sericata tissue libraries.
Matching of the counts to miRBase V19 identified known annotated miRNAs from insect and mammals. The counts per matched miRNA as a proportion of all counts that matched to miRBase are shown in the four tissues. Species nomenclature is used for the miRNA with the perfect matches to the L. sericata reads: dme, Drosophila melanogaster (fruit fly); tca, Tribolium castaneum (red flour beetle); sha, Sarcophilus harrisii (Tasmanian Devil); dps, Drosophila pseudoobscura (fruit fly); aae, Aedes aegypti (Mosquito).
Fig 4.
Droplet digital RT-PCR validation of small RNAs in tissue samples and through L. sericata developmental stages.
A. Assessment of small RNA abundances in dissected tissues, instar 1 ES and whole instar 1 larvae and B. throughout developmental stages. Average of replicates (for ES and developmental stages) plotted and error bars are standard deviations.