Table 1.
List of genes and primers used in the study for quantitative PCR analyzed by quantitative real-time RT-PCR analysis.
Fig 1.
Survival of A. pisum aphids after bacterial ingestion.
Graph show the survival of A. pisum aphids infected with S. marcescens Db11 (red), S. symbiotica CWBI-2.3T (green) and uninfected aphids (blue). Error bars represent ± 95% CI.
Fig 2.
Whole mount fluorescence in situ hybridization of S. symbiotica-infected A. pisum 0 day post-ingestion (A), 5 days post-ingestion (B) and 10 days post-ingestion (C).
Red Cy3 signals are S. symbiotica CWBI-2.3T, Green Cy5 signals are B. aphidicola and blue SYTOX Green signals are host tissues. (A) Red fluorescence in the midgut indicates ingestion of S. symbiotica CWBI-2.3T after feeding of contaminated diet. (B and C) 5 days and 10 days post-challenge, red fluorescence is visible in the midgut as well as in the entire intestine.
Fig 3.
Infection dynamic of S. symbiotica CWBI-2.3T during a period of ten days post-challenge.
S. symbiotica CWBI-2.3T densities are expressed in terms of DnaK gene copies per ef1-α gene copy. Points and bars indicate means and standard errors. Points sharing the same letters are not significantly different (p > 0.05) using post-hoc Tukey’s tests.
Fig 4.
Quantitative real-time RT-PCR analysis of transcriptional levels of six selected A. pisum genes in response to bacterial infection.
The mRNA levels of selected genes in infection with S. symbiotica CWBI-2.1T (green), S. marcescens Db11 (red) were determined and are shown relative to their expression levels in untreated insects (blue). The expression level was normalized by comparative CT (ΔΔCT) method using ef1-α as reference gene. Columns and bars indicate means and standard errors.