Fig 1.
Overview of the tryptophan metabolism and the kynurenine pathway.
IDO-1: Indoleamine 2,3-dioxygenase 1. IDO-2: Indoleamine 2,3-dioxygnease 2. TDO2: Tryptophan 2,3-dioxygenase. KATs: Kynurenine amino transferases. KMO: Kynurenine-3-monooxygenase. KYNU: Kynureninase. 3HAO: 3-hydroxyanthranilate oxygenase
Fig 2.
Immunodetection of l-kynurenine in colorectal and breast tumour specimens.
A and B, Representative micrographs of immunohistochemical stainings of paraffin-embedded healthy epithelia and colorectal or breast cancer samples using specific antibodies targeting kynurenine (clone 3D4-F2). On the right panel, graph represents kynurenine immunoscore (obtained from 2 independent TMA cores) with % of Kynurenine positive patients. (C) Representative micrographs of kynurenine immunostainings of paraffin-embedded breast cancer microenvironment.
Fig 3.
Immunodetection of IDO-1 and l-Kynurenine in colorectal cancer samples.
A and B, Representative micrographs of immunohistochemical stainings of paraffin-embedded colorectal cancer samples using specific antibodies targeting IDO-1 or l-kynurenine. On the up-right panel, graph represents IDO-1 immunoscore (obtained from 2 independent TMA cores) with % of IDO-1 positive patients. On the downright panel, scatter plot represents IDO-1 immunoscore over kynurenine immunoscore (C) Representative micrographs of IDO-1 and kynurenine immunostainings of immune cells from paraffin-embedded colorectal cancer samples.