Fig 1.
Whole tick sections subjected to ISH assay.
(A) Nymph with non-apparent EML infection. (B) Female basis capitulum showing heavy infection of epithelial tissues. (C) Male synganglion showing heavy infection of outer cortex. (D) Transversal section through male basis capitulum with cheliceral sheathes shown at left. Red indicates bacterial probe hybridization, blue indicates nuclear staining. Scale bars (SB) A, B, C = 10μm, D = 5 μm.
Fig 2.
Whole tick sections subjected to ISH assay.
(A) Acini and salivary ducts of infected adult female SB = 1μm. (B) Spermatozoa of adult male with infected lining sheath. SB = 1μm. (C) Infected adult male accessory gland (single lobe). SB = 2μm. (D) Infected adult male accessory gland (multiple lobes). SB = 10μm.
Fig 3.
Whole tick sections subjected to ISH assay.
Salivary gland of adult male showing multiple infected acini and salivary ducts. SB = 2μm.
Fig 4.
Whole tick sections subjected to ISH assay.
(A) Infected epithelial cells lining tracheole in adult male (bright field microscopy). (B) Same specimen as (A) imaged using fluorescent red filter. (C) Appendage of infected adult male. Arrows indicate hemocytes. SB = 2μm.
Fig 5.
(A) Male salivary gland showing Type I and Type II acini. Arrows indicate EML morulae. Inset shows morula at higher magnification. (B) Synganglion of infected adult male tick. Np = neuropile, nsg = neurosecretory granules. Inset shows morulae at higher magnification. (C) Infected epithelial sheath surrounding a tracheole. (D) Adult male accessory gland with heavy infection of lobe on the right. SB A,B,D = 5μm, C = 1μm.